Validation results for sgRNAs that were highlighted in the pooled CRISPR screens and selected for further characterization.

For each SNP, we characterized the sgRNA with the best overall performance in the CRISPR screens. For each sgRNA, we combined results from 3–4 biological replicates. Within each replicate, we compared the overall distributions of the endothelial cell readouts (between SNP sgRNA and negative control sgRNA) using the Kolmogorov-Smirnov test (Fig 3B). To combine results across replicates and assess significance, we used a simple one-tailed Student’s t-test. MFI, mean fluorescence intensity from the flow cytometer. (XLSX)