Identifying Human Specific Adverse Outcome Pathways of Per- and Polyfluoroalkyl Substances Using Liver-Chimeric Humanized Mice

We investigated the effects of per- and polyfluroralkyl substance exposure on FRG liver-chimeric humanized mice on a C57Bl/6 background. These PFAS included PFOA, PFOS, and GenX. These mice were exposed to water ad libitum for 28 days to reach human relevant serum concentrations. We then performed RNA-Sequencing on isolated whole liver lysate to determine differentially expressed genes. Overall design: Comparative gene expression profiling analysis of RNA-seq data for PFOA, PFOS, and GenX (n of 3 per group) exposed humanized mice and compared to the control group. cDNA was sequenced using 2 lanes targeting 25 million reads/sample.