Supplementary data of MSI analysis

Detailed knowledge of tissue-specific metabolic profiles of the lung is vital for elucidating the molecular pathological signature of lung aging and exploring potential metabolic targets to improve aging lung function. In this study, a spatially resolved metabolomics approach based on airflow-assisted desorption electrospray ionization mass spectrometry imaging (AFADESI–MSI) was proposed to investigate tissue-specific metabolic disturbances in the lungs of naturally aging mice. This dataset holds considerable potential for exploring the metabolic reprogramming of the aging lung, with important value for understanding the underlying mechanisms of aging-associated lung diseases.