The 3’ Untranslated Region (3’UTR) Coding Sequence of PHO84, Rather Than the Antisense RNA, Promotes Gene Repression

We provide evidence that the model of antisense-mediated repression of PHO84 gene is not correct, and that the 3’UTR of PHO84 exhibits a repressive effect on the sense transcript. We also provide a list of trans-acting elements promoting 3’UTR-dependent repression. Such information can be taken further to possibly identify key players in human diseases arising from gene expression dysregulation. Comparative gene expression profiling analysis of Transient transcriptome sequencing (TT-seq) data was performed for wild-type and dbp2 KO S. cerevisiae strains