Amaranthus hypochondriacus strain:cultivar Revancha Transcriptome or Gene expression

Total RNA was obtained from leaves (A. hypochondriacus cv. Revancha) or pigmented stems (A. hypochondriacus India 38040). Different sources of RNA were used to generate the six cDNA libraries employed for the pyrosequencing runs: i) leaves of intact plants grown under natural greenhouse conditions in the summer of 2009 (Source 1, S1) ; ii) pooled damaged leaf tissue from plants subjected to herbivory for 1, 4 and 12 h (¡Ö 20% maximum leaf-tissue loss) by larvae of the salt marsh caterpillar Estigmene acrea (S2); iii ) leaves of plants showing visible signs of water-stress after withholding watering for 3 days (S3), and iv) of plants subjected to the salt-stress produced by watering the plants for three straight days with 100 ml of a 400 mM NaCl solution (S4). Leaf material was also obtained from leaves of plants infected with Pseudomonas argentinensis, a bacterial amaranth pathogen (S5) and from pigmented (red) stem tissue of un-stressed 38040 plants (S6).