Data_Sheet_3_“CATAStrophy,” a Genome-Informed Trophic Classification of Filamentous Plant Pathogens – How Many Different Types of Filamentous Plant Pathogens Are There?.XLSX

The traditional classification of fungal and oomycete phytopathogens into three classes – biotrophs, hemibiotrophs, or necrotrophs – is unsustainable. This study highlights multiple phytopathogen species for which these labels have been inappropriately applied. We propose a novel and reproducible classification based solely on genome-derived analysis of carbohydrate-active enzyme (CAZyme) gene content called CAZyme-Assisted Training And Sorting of -trophy (CATAStrophy). CATAStrophy defines four major divisions for species associated with living plants. These are monomertrophs (Mo) (corresponding to biotrophs), polymertrophs (P) (corresponding to necrotrophs), mesotrophs (Me) (corresponding to hemibiotrophs), and vasculartrophs (including species commonly described as wilts, rots, or anthracnoses). The Mo class encompasses symbiont, haustorial, and non-haustorial species. Me are divided into the subclasses intracellular and extracellular Me, and the P into broad and narrow host sub-classes. This gives a total of seven discrete plant-pathogenic classes. The classification provides insight into the properties of these species and offers a facile route to develop control measures for newly recognized diseases. Software for CATAStrophy is available online at https://github.com/ccdmb/catastrophy. We present the CATAStrophy method for the prediction of trophic phenotypes based on CAZyme gene content, as a complementary method to the traditional tripartite “biotroph–hemibiotroph–necrotroph” classifications that may encourage renewed investigation and revision within the fungal biology community.

传统的真菌和卵菌类植物病原菌被划分为三大类——生物寄生物、半生物寄生物或坏死寄生物——这一分类体系已显不适应性。本研究突出了多个植物病原菌物种，其分类标签已被错误地应用。我们提出了一种新颖且可重复的分类方法，该方法仅基于碳水化合物活性酶（CAZyme）基因含量进行基因组分析，称为CAZyme辅助的训练与分类系统（CATAStrophy）。CATAStrophy将与活植物相关的物种划分为四大主要类别：单聚营养型（Mo）（对应生物寄生物）、多聚营养型（P）（对应坏死寄生物）、中聚营养型（Me）（对应半生物寄生物）和维管营养型（包括通常被描述为萎蔫、腐烂或黑斑病的物种）。Mo类别包括共生体、吸器和非吸器物种。Me分为细胞内和细胞外中聚营养型，而P分为广宿主亚类和窄宿主亚类。由此，总共有七个独立的植物致病类别。该分类体系为这些物种的特性提供了洞察，并提供了开发针对新识别疾病控制措施的便捷途径。CATAStrophy软件可在https://github.com/ccdmb/catastrophy在线获取。我们提出基于CAZyme基因含量的CATAStrophy方法，用于预测营养表型，作为传统三分法“生物寄生物-半生物寄生物-坏死寄生物”分类的补充，可能激发真菌生物学领域的重新研究和修订。