Enterovirus 71 oncolysis of malignant gliomas

This study finds that EV71 has oncolytic activity against experimental human malignant gliomas. RNA-seq analysis of infected glioma cells reveals transcriptional up-regulation, notably genes in apoptosis pathways. Application of virus targeting based on brain-specific microRNA-124 enhances the safety profile. Oncolysis with EV71 may be a potentially novel treatment for malignant gliomas. Overall design: To investigate the impact of EV71 infection on glioma cells at the molecular level, we carried out transcriptome analyses (RNA-seq). Total RNA samples from CCF-STTG1 glioma cells after EV71 or mock infection were used for RNA-seq analysis by using Illumina HiSeqTM 2000 System.