Genomic occupancy of TFs ISL1 and NKX2.5 in cardiac and neural populations

ChIP-sequencing demonstrated that ISL1 binds cardiac DNA in a cell-type-specific manner, and that NKX2.5 is both necessary and sufficient for this localization. Overall design: 3 replicates of ISL1-bound DNA (ChIP-seq) in WT and ISL1–/– hiPSC derived d6 CPs. 3 replicates of NKX2.5 ChIP-seq in WT NKX2.5–/– hiPSC derived d6 CPs. 3 replicates of ISL1 ChIP-seq in NKX2.5–/– hiPSC derived d6 CPs. 3 replicates of ISL1 ChIP-seq in WT and ISL1–/– hiPSC derived d18 MNPs. One replicate of ISL1 and NKX2.5 ChIP-seq in adCMV::NKX2.5-infected d18 MNPs. One replicate of NKX2.5 ChIP-seq in adCMV::EGFP-infected d18 MNPs.