IC50 values for nocuolin A anti-proliferative activity against nine human cancer cell lines at 72 h.

Metabolic activity was assessed using colorimetric PrestoBlue assay and the cell membrane permeability was measured by fluorescence probes Hoechst 33342 and Ethidium homodimer (Methods). The most sensitive and least sensitive cell lines were the glioma cancer cell lines U251 and U87, respectively. IC50 values equal to or below 1 μM are highlighted in bold. The p53 status is indicated as WT (wild type) or M (mutated); number (codon position) is followed with the sequence of WT/mutated codon, ins3 (insertion of 3 bases). The p53 status was adopted from IACR database (http://p53.iarc.fr/CellLines.aspx) and p53 Web Site (http://p53.free.fr/index.html). Values in the last column refer to the cell doubling time (h).