Transcriptome-wide mapping of RNA:protein interactions of SRSF6 in HeLa cells grown under normoxic and hypoxic conditions by iCLIP

We used HeLa cells that express GFP-tagged SRSF6 at physiological levels and subjected them to 4 h or 24 h hypoxia (0.2% oxygen). Cells grown in normal conditions were used as control (Normoxia, 21% oxygen). We performed iCLIP using anti-GFP antibodies and compared the binding pattern of SRSF6 between normoxia, 4h hypoxia and 24h hypoxia. Overall design: 3 biological replicates per condition were subjected to the iCLIP procedure with UV-crosslinking at 254 nm. For IPs either an anti-GFP antibody was used. No UV treatment served as negative control.