Inhibition of odorant and Orco agonist initiated current responses of oocytes expressing insect odorant receptors by various amines

Figure 2C Data. Current responses of oocytes expressing Cqui\Orco to 30µM OLC12 (~EC5) in the presence of 100µM of each compound presented as a percentage of the average of two preceding responses to OLC12 alone. Sham is an application of ND96 (no antagonist candidate).Figure 3A Data. Current responses of oocytes expressing Cqui\Orco to 30µM OLC12 (~EC5) in the presence of various concentrations of tryptamine, tyramine or phenethylamine are presented as a percentage of the response to OLC12 alone.
Figure 3B Data_30µM OLC12 curve. Current responses of oocytes expressing Cqui\Orco to 30µM OLC12 in the presence of various concentrations of tryptamine are presented as a percentage of the response to OLC12 alone.
Figure 3B Data_100µM OLC12 curve. Current responses of oocytes expressing Cqui\Orco to 100µM OLC12 in the presence of various concentrations of tryptamine are presented as a percentage of the response to OLC12 alone.
Figure 4A Data. Oocytes expressing Cqui\Orco, Agam\Orco or Dmel\Orco were activated by OLC12 (Cqui\Orco, 30µM; Agam\Orco, 30µM; Dmel\Orco, 20µM). Values are the current responses in the presence of 10µM tryptamine presented as a percentage of the average of two preceding responses to OLC12 alone.
Figure 4B Data. Oocytes expressing Agam\Orco+Agam\Or65 were activated by 100nM eugenol, oocytes expressing Onub\Orco+Onub\Or6 were activated by 1µM Z11-14:OAc, oocytes expressing Mcar\Orco+Mcar\Or5 were activated by 150µM 2-phenylethanol. Values represent the normalized (see Methods) percentage of current responses in the presence of 10µM tryptamine to the preceding response to odorant alone.
Figure 4C Data. Oocytes expressing Agam\Orco+Agam\Or65 were activated by 100nM eugenol, oocytes expressing Onub\Orco+Onub\Or6 were activated by 1µM Z11-14:OAc, oocytes expressing Mcar\Orco+Mcar\Or5 were activated by 150µM 2-phenylethanol. Values represent the normalized (see Methods) percentage of current responses in the presence of 100µM tyramine to the preceding response to odorant alone.
Figure 5A Data_OLC12 20µM curve. Current responses of oocytes expressing Agam\Orco+Agam\Or65 to 20µM OLC12 in the presence of various concentrations of tryptamine are presented as a percentage of the response to OLC12 alone.
Figure 5A Data_OLC12 100µM curve. Current responses of oocytes expressing Agam\Orco+Agam\Or65 to 100µM OLC12 in the presence of various concentrations of tryptamine are presented as a percentage of the response to OLC12 alone.
Figure 5B Data_Eugenol 10nM curve. Oocytes expressing Agam\Orco+Agam\Or65 were activated by 10nM eugenol. Values are the normalized (see Methods) percentage of current responses in the presence of various concentrations of tryptamine compared to the preceding response to odorant alone.
Figure 5B Data_Eugenol 100nM curve. Oocytes expressing Agam\Orco+Agam\Or65 were activated by 100nM eugenol. Values are the normalized (see Methods) percentage of current responses in the presence of various concentrations of tryptamine compared to the preceding response to odorant alone.
Figure 6A Data. Values represent the normalized (see Methods) percentage of current responses of oocytes expressing Agam\Orco+Agam\Or27 (activated by 3µM L-fenchone), Agam\Orco+Agam\Or28 (activated by 40µM acetophenone), Agam\Orco+Agam\Or31 (activated by 70µM geranyl acetate), Agam\Orco+Agam\Or39 (activated by 10µM 6-methyl-5-hepten-2-one), Agam\Orco+Agam\Or48 (activated by 3µM 2-nonanone), or Agam\Orco+Agam\Or65 (activated by 100nM eugenol) in the presence of 10µM tryptamine, as compared to the preceding response to odorant alone.
Figure 6B Data. Values represent the normalized (see Methods) percentage of current responses of oocytes expressing Agam\Orco+Agam\Or27 (activated by 3µM L-fenchone), Agam\Orco+Agam\Or28 (activated by 40µM acetophenone), Agam\Orco+Agam\Or31 (activated by 70µM geranyl acetate), Agam\Orco+Agam\Or39 (activated by 10µM 6-methyl-5-hepten-2-one), Agam\Orco+Agam\Or48 (activated by 3µM 2-nonanone), or Agam\Orco+Agam\Or65 (activated by 100nM eugenol) in the presence of 10µM tyramine, as compared to the preceding response to odorant alone.

图2C数据：展示表达CquiOrco的卵母细胞对30µM OLC12（约EC5）的当前响应，在每种化合物浓度为100µM的条件下，以相对于单独使用OLC12的前两次响应平均值的百分比表示。安慰剂组为ND96（无拮抗剂候选物）的应用。图3A数据：展示表达CquiOrco的卵母细胞对30µM OLC12（约EC5）的当前响应，在色胺、酪胺或苯乙胺的各种浓度存在下，以相对于单独使用OLC12的响应百分比表示。图3B数据_30µM OLC12曲线：展示表达CquiOrco的卵母细胞在色胺各种浓度存在下对30µM OLC12的当前响应，以相对于单独使用OLC12的响应百分比表示。图3B数据_100µM OLC12曲线：展示表达CquiOrco的卵母细胞在色胺各种浓度存在下对100µM OLC12的当前响应，以相对于单独使用OLC12的响应百分比表示。图4A数据：表达CquiOrco、AgamOrco或DmelOrco的卵母细胞被OLC12激活（CquiOrco，30µM；AgamOrco，30µM；DmelOrco，20µM）。数值为在10µM色胺存在下的当前响应，以相对于单独使用OLC12的前两次响应平均值的百分比表示。图4B数据：表达AgamOrco+AgamOr65的卵母细胞被100nM丁香油酚激活，表达OnubOrco+OnubOr6的卵母细胞被1µM Z11-14:OAc激活，表达McarOrco+McarOr5的卵母细胞被150µM 2-苯乙醇激活。数值代表在10µM色胺存在下，相对于先前的嗅觉刺激单独响应的标准化（见方法）的当前响应百分比。图4C数据：表达AgamOrco+AgamOr65的卵母细胞被100nM丁香油酚激活，表达OnubOrco+OnubOr6的卵母细胞被1µM Z11-14:OAc激活，表达McarOrco+McarOr5的卵母细胞被150µM 2-苯乙醇激活。数值代表在100µM酪胺存在下，相对于先前的嗅觉刺激单独响应的标准化（见方法）的当前响应百分比。图5A数据_OLC12 20µM曲线：展示表达AgamOrco+AgamOr65的卵母细胞在色胺各种浓度存在下对20µM OLC12的当前响应，以相对于单独使用OLC12的响应百分比表示。图5A数据_OLC12 100µM曲线：展示表达AgamOrco+AgamOr65的卵母细胞在色胺各种浓度存在下对100µM OLC12的当前响应，以相对于单独使用OLC12的响应百分比表示。图5B数据_丁香油酚10nM曲线：表达AgamOrco+AgamOr65的卵母细胞被10nM丁香油酚激活。数值为在色胺各种浓度存在下，相对于先前的嗅觉刺激单独响应的标准化（见方法）的当前响应百分比。图5B数据_丁香油酚100nM曲线：表达AgamOrco+AgamOr65的卵母细胞被100nM丁香油酚激活。数值为在色胺各种浓度存在下，相对于先前的嗅觉刺激单独响应的标准化（见方法）的当前响应百分比。图6A数据：数值代表表达AgamOrco+AgamOr27（由3µM L-桉树脑激活）、AgamOrco+AgamOr28（由40µM乙酸苯甲醇激活）、AgamOrco+AgamOr31（由70µM香叶基乙酸激活）、AgamOrco+AgamOr39（由10µM 6-甲基-5-庚烯-2-酮激活）、AgamOrco+AgamOr48（由3µM 2-壬酮激活）或AgamOrco+AgamOr65（由100nM丁香油酚激活）的卵母细胞在10µM色胺存在下的当前响应的标准化（见方法）百分比，与先前的嗅觉刺激单独响应相比。图6B数据：数值代表表达AgamOrco+AgamOr27（由3µM L-桉树脑激活）、AgamOrco+AgamOr28（由40µM乙酸苯甲醇激活）、AgamOrco+AgamOr31（由70µM香叶基乙酸激活）、AgamOrco+AgamOr39（由10µM 6-甲基-5-庚烯-2-酮激活）、AgamOrco+AgamOr48（由3µM 2-壬酮激活）或AgamOrco+AgamOr65（由100nM丁香油酚激活）的卵母细胞在10µM酪胺存在下的当前响应的标准化（见方法）百分比，与先前的嗅觉刺激单独响应相比。