Effect of RIPK2 knockdown on U118 cells related behavior

IFN-Ⅰ plays an important role in the progression of LGG. We used a set of genes from MsigDB associated with interferon alpha responses, bioinformatic screening based on public databases, and validation of internal and external data to obtain relevant biomarkers. Immunohistochemical results of clinical samples showed that the higher the grade of glioma, the higher the expression of RIPK2 gene. Subsequently, U118 knockdown RIPK2 cell line was constructed, and the knockdown group could inhibit the proliferation of tumor cells and promote apoptosis. RNA-seq sequencing results showed that, compared with the control group, GO enrichment analysis showed that the differential genes were mainly concentrated in bioregulatory and molecular function related pathways such as ion gated channel activity and cell adhesion, and KEGG enrichment analysis showed that, Genes were mainly enriched in inflammatory pathways such as NF-kappa B signaling pathway, TNF signaling pathway and IL17 signaling pathway. RNA-seq profiling of wildtype U118 cells and their knockdown derivatives (siRIPK2_1, siRIPK2_2, siRIPK2_3, siRIPK2_4) at Day2 of siRNA transfection