Location based fluorescent receptor binding assay (RBAF) results list the date of sampling, number of lionfish screened and the number of positive samples.

Following the RBAF screening, all the positive samples (n = 30) plus a subset of negative samples (n = 24) were next analyzed using CBA-N2a and LC-MS/MS. The negative samples identified by RBAF were also negative when measured by CBA-N2a and LC-MS/MS. CBA-N2a and LC-MS/MS confirmed that a subset of the samples screened contained measurable levels of C-CTX-1. Location abbreviations FGBNMS and USVI stand for Flower Garden Banks National Marine Sanctuary and United States Virgin Islands, respectively.