The expression profile of miRNA in the bladder tissue of partial bladder outlet obstruction rats before and after treatment of human urine-derived stem cells

Obstruction in any part between the outlet of the bladder and the external urethra is called partial Bladder outlet obstruction (pBOO), which is a common disease in urology, which may cause bladder dysfunction. We have comfirmed that human urine-derived stem cells (hUSCs) could improve the bladder function of pBOO rats in our previous study. We thus explored the role of miRNAs in pBOO rats before and after treatment of hUSCs, and revealed that they could serve as effective prevention and treatment therapeutic targets. Rat bladder tissues were used for miRNA sequencing. We identified 8 differentially expressed miRNAs in the pBOO rats before and after treatment of hUSCs. Bladder tissues miRNA profiles of partial bladder oublet obstruction rat before and after 12 weeks of treatment of human urine-derived stem cells were generated by high throughput sequencing using Illumina Hiseq 3000. Model 1 means a pBOO rat without USCs treated, while model 2 means another rat, comparing to Ctrl rats that means normal SD rats.