Effect of RPD3 phosphorylation on gene expression in glucose and sucrose

Effect of RPD3 phosphorylation on gene expression in glucose and sucrose Total RNA was isolated from exponential growing yeast cells by standard phenol-chloroform extraction procedures and the quality of RNA was examined using Agilent Bioanalyzer according to the manufacturer’s instructions. Library construction, sequencing and bioinformatics analysis were performed . There are three biological replicates for WT and Rpd3-2SA mutants in glucose and sucrose treatment conditions. Differential expression levels of aligned sequences were calculated using significant thresholds set at fold change over 1.5 and adjusted P-value < 0.05