Characterization of the effect of deleting the Nrg-1 (neuregulin-1) gene, using the endothelial driver line Tie2-Cre in E (embryonic day) 9.5 hearts

To investigate the role of Nrg-1 in heart ventricular chamber development we deleted Nrg-1 in endothelial lineages, harvested hearts at E9.5, and determined gene expression profiles, using RNA-seq.为探究神经调节蛋白1（Nrg-1）在心脏心室腔发育中的作用，我们在内皮细胞谱系中敲除Nrg-1，于胚胎发育第9.5天（E9.5）收取心脏组织，并通过RNA测序（RNA-seq）分析基因表达谱。