Data_Sheet_1_Integrative Description of Cryptic Tigriopus Species From Korea Using MALDI-TOF MS and DNA Barcoding.PDF

MALDI Time-of-Flight Mass Spectrometry (MALDI-TOF MS) provides a fast and reliable alternative method for species-level identification of pathogens and various metazoans. Compared to the commonly used mitochondrial cytochrome c oxidase subunit I (mtCOI) barcoding, advantages of MALDI-TOF MS are rapid species identifications and low costs. In this study, we used MALDI-TOF MS to determine whether spectra patterns of different species can be used for species identification. We obtained a total of 138 spectra from individual specimens of Tigriopus, which were subsequently used for various cluster analyses. Our findings revealed these spectra form three clear clusters with high AU value support. This study validates the viability of MALDI-TOF MS as a methodology for higher-resolution species identification, allowing detection of cryptic species of harpacticoida. In addition, we propose a new species, Tigriopus koreanus sp. nov. by utilizing integrative methods such as morphological comparison, mtCOI barcoding, and MALDI-TOF MS.

基质辅助激光解吸飞行时间质谱法（MALDI-TOF MS）提供了一种快速且可靠的病原体及各类多细胞生物物种水平鉴定方法。相较于常用的线粒体细胞色素c氧化酶亚基I（mtCOI）条形码，MALDI-TOF MS的优势在于物种鉴定的快速性和低成本。在本研究中，我们运用MALDI-TOF MS技术，旨在探究不同物种的光谱模式是否可用于物种鉴定。我们从Tigriopus个体的标本中获取了共计138个光谱，这些光谱随后被用于多种聚类分析。我们的研究结果表明，这些光谱形成了三个具有高AU值支持的清晰聚类。本研究验证了MALDI-TOF MS作为高分辨率物种鉴定方法的有效性，并允许检测到隐生物种的桡足类。此外，我们通过形态比较、mtCOI条形码和MALDI-TOF MS等综合方法，提出了一个新的物种——韩国虎虾（Tigriopus koreanus sp. nov.）。