Real-time quantitative PCR analysis of microRNAs in serum. Real-time quantitative PCR analysis of microRNAs in serum

Human serum samples were collected from patients with history of three or more recurrent spontaneous abortion and non- pregnant women with two or more successful deliveries. Chlamydia trachomatis infection was detected in the samples collected from the patients and real time PCR was perform to study the expression of microRNA genes in these patients. Overall design: qPCR gene expression profiling of microRNAs 101-3p, 133a, 146b-5p, 320b, 24, 559 genes in serum samples