MOESM2 of Molecular dissection of the replication system of plasmid pIGRK encoding two in-frame Rep proteins with antagonistic functions
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https://springernature.figshare.com/articles/MOESM2_of_Molecular_dissection_of_the_replication_system_of_plasmid_pIGRK_encoding_two_in-frame_Rep_proteins_with_antagonistic_functions/10302788/1
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Additional file 2: Table S1. Determination of pRK-1 plasmid copy number (PCN) in E. coli DH5α strain. Plasmid copy number was defined for three independent clones (1–3). For each of DNA isolate three QPCR reactions were performer (the table contains average values). Figure S2. Raw data from construction of standard curves and QPCR of total DNA preparates from E. coli DH5α clones harboring pRK-1. DNA isolated using QIAamp DNA Mini Kit (Qiagen). Figure S3. Raw data from construction of standard curves and QPCR of total DNA preparates from E. coli DH5α clones harboring pRK-1. DNA isolated by thermal lysis. Cells were suspended in water, boiled and centrifuged (supernatant used as a template). Figure S4. Raw data from construction of standard curves and QPCR of total DNA preparates from E. coli DH5α clones harboring pRK-1. Washed cells added directly to the PCR reaction.
提供机构：
Paweł Wawrzyniak
创建时间：
2019-11-14
