Figure S1 - Protective Efficacy and Immunogenicity of a Combinatory DNA Vaccine against Influenza A Virus and the Respiratory Syncytial Virus

HA-specific CD4+ T-cell responses. Balb/c mice were immunized according to Table 1. Since the low dose regimen did not result in substantial responses, only the groups which received a total of 40 µg of plasmid DNA were included. A) IAV-HA-specific CD4+ T-cell responses were analyzed one week after the second immunization by intracellular staining for the inflammatory cytokines IFN-γ, TNF and IL-2. The percentages of the different populations among the total CD4+ T-cells are shown. Mean values and standard errors of the means (SEM) represent 8 mice per vaccine group out of two independent experiments and 4 mice for the naïve group. (*** = p (PDF)