Technical and biological variation in the faecal metaproteomic analysis of subjects A, B and C.

Sample similarities are expressed as Pearson correlations, which were calculated for peptide feature intensities between different sample preparation replicates (experimental), different time point (temporal), and subjects (A, B, C) (ND: not determined; SD: standard deviation; n: number of comparisons). Column “A, B” presents data from an experiment in which the metaproteome of subjects A and B time point one were analysed and column “A, B, C” shows data of a repetition of those samples and an addition of a second time point and subject C. For detailed experimental setup see “Material and Methods” Figure S1.