1. Next Generation Sequencing Assessment of the Influence of Antibiotic Exposure on Bacterial Genome using a Hollow Fiber Culture System

Antibiotic resistance represents a global threat to public health. This project uses the hollow fiber culture model that provides computer-controlled flow of pharmacokinetically accurate antibiotic drug concentrations through and diffusing from the inner lumen of capillary fibers to expose extra luminal bacteria with the goal of assessing emergent antibiotic resistance dynamics and investigating factors that contribute to emergent resistance. In previous studies we observed the rapid emergence of functional antibiotic resistance. Whole genome shotgun sequencing was applied in this project to determine if genome variation was relevant to emergent resistance.