APPENDIX 3 in The Amidella clade in Europe (Basidiomycota: Amanitaceae): clarification of the contentious Amanita valens (E. - J. Gilbert) Bertault and the importance of taxon-specific PCR primers for identification

APPENDIX 3. — Usefulness of the taxon-specific primers for the generation of PCR amplicons from herbarium specimens’ DNA. ITS-I and ITS-II represent the first and second nrDNA intron, respectively, and LSU represents the proximal region of the 26S nrDNA. a, https://sites.duke.edu/vilgalyslab/rdna_primers_for_fungi (accessed on May 15 th 2021). TaxonSpecimenITS-IITS-IILSUCommentAmanita lepiotoidesG08ITS1F- Ale Ir1Ale If1-ITS4BaLR0R- Ale Lr1–f. subcylindrosporaAle Lf1-LR3aAle Lf1-LR5Amanita ponderosaG73Apo If1-ITS2_KYO2Apo If2-ITS4NLC2R- Apo Lr1–f. valensAmanita pseudovalens G74SR6Ra- Aps Ir1Aps If1-NLC2Aps Lf1- Aps Lr2–comb. nov.MPU1ITS1F- Aps Ir1Aps If1-ITS4LR0R- Aps LR1All regions afterNLC2R- Aps Lr2reamplificationMPU2SR6R- Aps Ir1Aps If1-ITS4Aps Lf1- Aps Lr2ITS-II region afterreamplification