Peptides.

Sequences of BH3 containing peptides used for pull down experiments are shown. Each peptide was biotinylated at the N-terminus.
For generation of antibodies for phosphorylated serine 117 (S117), phospho- and non-phospho derivatives were synthesised of the BAK sequence surrounding S117. An N-terminal cysteine was added to enable coupling to resin for column purification of phosphor-specific antibodies.