Original paired-end transcriptome outputs from the RNAseq analyses of the deep-sea zoanthid Zibrowius primnoidus

This dataset contains outputs generated from the original paired-end transcriptomic analyses of the deep-sea zoanthid Zibrowius primnoidus. The dataset includes the following files:

• 155835_ID3533_1-1_S111_L004_R1_001.fastq.gz.P.qtrim.gz: Preprocessed forward reads used for the de novo assembly, obtained from paired-end RNA-Seq data of Zibrowius primnoidus, containing high-quality sequences that passed quality trimming and filtering with Trimmomatic.

• 155835_ID3533_1-1_S111_L004_R2_001.fastq.gz.P.qtrim.gz: Preprocessed reverse reads used for the de novo assembly, obtained from paired-end RNA-Seq data of Zibrowius primnoidus, containing high-quality sequences that passed quality trimming and filtering with Trimmomatic.

• Zibrowius_primnoidus_RF_assembly.Trinity.fasta: Original, non-filtered de novo paired-end transcriptome assembly of Zibrowius primnoidus, generated from ~90 million PE reads using the Trinity assembler.

• Zibrowius_primnoidus_RF_assembly.Trinity.fasta_stats.txt: Statistical summary of the paired-end transcriptome assembly of Zibrowius primnoidus (e.g. number of transcripts, N50, GC content).

• Zibrowius_primnoidus_RF_assembly.Trinity.fasta.gene_trans_map: Transcript-to-gene mapping file generated during the Trinity paired-end transcriptome assembly of Zibrowius primnoidus.

• quant_Zp.sf: Salmon output file containing expression estimates (transcript-level abundance) for the assembled transcripts from the paired-end RNA-Seq data of Zibrowius primnoidus.

Additionally, the dataset includes the following files from DIAMOND BLASTx analyses, which used the original de novo paired-end transcriptome assembly of Zibrowius primnoidus:

• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6: BLASTx output file against the UniRef90 database, reporting the top alignment for each query (assembled transcript) from the paired-end assembly of Zibrowius primnoidus.

• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6.grouped: Grouped BLASTx hits from the paired-end assembly of Zibrowius primnoidus, designed to improve sequence coverage by combining multiple high-scoring segment pairs (HSPs).

• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6.hist: Histogram summarizing the distribution of BLASTx hit lengths obtained from the paired-end assembly of Zibrowius primnoidus.

• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6.w_pct_hit_length: File providing percentages of hit lengths from BLASTx analyses of the paired-end assembly of Zibrowius primnoidus, including each top hit’s length and the percentage of that length covered in the alignment.