Genome-wide maps of JMJ28pro::JMJ28:3xFLAG in 14 days old arabidopsis
收藏NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE155623
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资源简介:
We report the application of single-molecule-based sequencing technology for high-throughput profiling of JMJ28 in 14 days old arabidopsis. By obtaining sequence from chromatin immunoprecipitated DNA, we generated genome-wide JMJ28-binding maps of 14 days old arabidopsis. To reveal bound genes by JMJ28, chimeric protein JMJ28-3xFLAG was expressed under JMJ28 promoter in jmj28 (JMJ28pro:JMJ28:3xFLAG/ jmj28). ChIP was performed using anti-FLAG antibody (FLAG-M2, F1804; SIGMA), and ChIP DNA were analyzed by Illumina Novaseq 6000. Chromatin immunoprecipitation was performed forJMJ28pro::JMJ28:3xFLAG-expressing Arabidopsis. ChIP DNA was analyzed by Illumina Novaseq 6000.
创建时间:
2021-03-30



