Single-day protein LC–MS bioanalysis: can next-generation trypsins cut it?: supplementary table 1

Aims: The drive toward more sensitive LC–MS assays has resulted in long, complex methods. We
assessed next-generation trypsins to identify a suitable candidate to integrate into protein LC–MS
method development strategies, to simplify methods and increase throughput. Materials & methods: The
performance of commercially available next-generation trypsins was assessed based on the digestion of
protein standards in buffer and complex matrix by LC–high-resolution MS. Results: The performance of
all next-generation trypsins assessed exceeded that of an overnight tryptic digest in a fraction of the
time. Performing reduction and alkylation prior to digestion with heat-stable trypsins may be beneficial
and should be investigated. Conclusion: Promega Rapid-Digestion