RNA seq to compare gene expression profiles between Npm1 WT and Npm1 knockdown cells
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https://www.ncbi.nlm.nih.gov/sra/SRP212657
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资源简介：
Mouse KrasG12D/P53-/-(KP) lung tumor cells were transfected with pLKO.1-Tet on-shNpm1-3(N3), pLKO.1-Tet on-shNpm1-4(N4) or pLKO.1-Tet on-shNpm1-5 (N5) and then selected by using 2ug/ml puromycin, to establish the 3 stable cell lines. These 3 stable cell lines (N3, N4 or N5) were treated with 100ng/ml Doxycline and then the total RNA of the treated and untreated cells were harvested for RNA seq. Overall design: Examination of differential gene expression profils in KP-shNpm1-3 (N3), KP-shNpm1-4 (N4) and KP-shNpm1-5 (N5) cells with or without Doxycycline treatment
创建时间：
2020-09-22
