NAMPT and NAPRT activities in the absence or presence of ATP.
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NAMPT (15 ng) and NAPRT (20 ng) were incubated with 20 µM [14C]Nam and [14C]NA for 30 min, respectively, in the presence of 1 µM PRPP (low substrate concentration) without or with 1 mM ATP. NAMPT (96 ng) and NAPRT (100 ng) were also incubated with 50 µM [14C]Nam and [14C]NA for 7 and 6 min, respectively, in the presence of 50 µM PRPP (high substrate concentration) without or with 1 mM ATP.
创建时间:
2015-12-02



