Giardia lamblia WB: 5'5npac control vs pPMBF1

Transcriptional profiling of Giardia lamblia WB comparing control 5'5npac cells with pPMBF1 cells. Multiprotein bridging factor 1 (MBF1) gene family proteins are involved in cell growth and differentiation in various organisms. We asked whether Giardia MBF1-like protein can induce various endogenous genes. We used epitope-tagged system to overexpress Giardia MBF1 by constructing and transfecting pPMBF1, a plasmid for expressing MBF1, to trophozoites. The control cell line is 5'5npac cell line, which expressed only the puromycin selection marker. To understand the effect of MBF1 overexpression on transcription profiles, we performed microarray analysis for pPMBF1 and 5'5npac cell lines. Two-condition experiment 5'5npac cells with pPMBF1 cells.