Supplementary file 2_An updated systematic review and meta-analysis of tooth loss in patients with periodontitis and the risk of mild cognitive impairment.docx

ObjectiveConflicting evidence has shown a connection between tooth loss, periodontitis, and increased risk of mild cognitive impairment (MCI); thus, we conducted a meta-analysis to clarify this association.
Materials and methodsPubMed, Embase, Web of Science, the Cochrane Library, and MEDLINE (up to March 2025) were searched. Cross-sectional and longitudinal studies were included. Fully-adjusted odds ratios (aORs) with the most severe form of periodontitis or tooth loss were pooled with a random effects model using STATA 18/MP software. Subgroup and sensitivity analyses were conducted to elucidate possible heterogeneity.
ResultsThe meta-analysis of five studies showed that neither periodontitis nor tooth loss substantially increased MCI risk after full adjustment, with pooled aORs of 1.27 (95% CI: 0.87–1.86, I2 = 63.9%) and 1.22 (95% CI: 0.97–1.54, I2 = 53.8%), respectively. A significant association was found between periodontitis and MCI with low heterogeneity in the Centers for Disease Control and Prevention-American Academy of Periodontology diagnostic criteria for periodontitis subgroup (aOR of 2.78, 95%CI 1.54–5.02, I2 = 0.0%) and between MCI and significant tooth loss (aOR of 1.50, 95%CI 1.15–5.95, I2 = 0.0%). The sensitivity result confirmed the robustness of the results.
ConclusionOverall, periodontitis and tooth loss were not statistically associated with increased MCI risk. Whereas the association between severe periodontitis and significant tooth loss and MCI was statistically significant. Thus, severe periodontitis and significant tooth loss were linked to decreased cognitive function.
Systematic Review RegistrationPROSPERO CRD420251055491.