Transcriptomic analyses of improved cold tolerance in cucumber (Cucumis sativus L.) caused by Exogenous chitosan oligosaccharide

Purpose: the goals of this study are to provide a theoretical basis for the use of chitosan oligosaccharide to alleviate the damage caused by cold stress in cucumber growth and development. The transcriptome sequence were generated by deep sequencing with three repeats using Illumina. The sequence reads that passed filters were mapped tocucumber genome (http://cucurbitgenomics.org/organism/18) using HISAT2 software. The differently expressed genes were identify by |log2(FoldChange)| > 0 & p < 0.05, and qRT–PCR validation was performed using SYBR Green assays