The effects of X-ray radiation on pancreatic cancer cells. The effects of X-ray radiation on pancreatic cancer cells

Dying pancreatic cancer cells from chemoradiation can directly stimulate repopulation of surviving cells. We used microarrays to conduct the analysis of whole transcript profiles in pancreatic cancer cells treated with X-ray radiation. Overall design: Pancreatic cancer cells were seeded into culture plate overnight with 2% FBS in culture medium and then were irradiated with 10Gy using an Oncor linear accelerator. Irradiated pancreatic cancer cells were collected at 24h after X-ray radiation for RNA extraction and hybridization on Affymetrix microarray. We sought to obtain whole transcript profiles in pancreatic cancer cells treated with X-ray radiation.