High-throughput differentiation of human blood vessel organoids reveals overlapping and distinct functions of the cerebral cavernous malformation proteins

We used single cell RNA sequencing to compare gene expression between WT, CCM1 knockout, CCM2 knockout and CCM3 knockout blood vessel organoids. Comparative gene expression profiling and cell cluster analysis of single cell RNA-seq data for wild-type and CCM1, CCM2 and CCM3 knockout blood vessel organoids differentiated from hiPSCs after gene inactivation with CRISPR/Cas9