Characterization of high mountain glacier microbiota following inundation with wildfire soot

Glacier ecosystems are shrinking at an accelerating rate due to changes in climate, but also due to increased deposition of soot, which has resulted in darkening of glacier surfaces and subsequent changes in absorption of light, associated heat, and altered microbial communities. In this study, compositions of microbial communities on surfaces of the Athabasca Glacier (Alberta, Canada) were measured by use of DNA metabarcoding. Three matrices, glacier ice, cryoconite hole, and glacial surface sediment, were analyzed. The samples were extracted for DNA and were PCR amplified targeting the hypervariable V3-V4 region of the 16S ribosomal RNA subunit I gene of prokaryotes, as well as the hypervariable V3 region of the 18S ribosomal RNA gene of eukaryotic organisms. Amplicons were sequenced performing a 600-cycle paired-end sequencing run on an Illumina® MiSeq sequencer. This dataset includes the demultiplexed sequencing output, the feature table with taxonomic annotation, and the sample metadata.

冰川生态系统正因气候变化而以日益加速的速度缩减，同时亦因烟灰沉积量的增加，导致冰川表面变暗，进而引起对光的吸收、相关热能的改变以及微生物群落的变迁。在本研究中，通过DNA条形码技术对加拿大艾伯塔省的阿萨巴斯卡冰川（Athabasca Glacier）表面微生物群落的组成进行了测量。分析了冰川冰、冷孔冰和冰川表面沉积物这三种基质。样本经过DNA提取并进行聚合酶链式反应（PCR）扩增，以靶向原核生物的16S核糖体RNA亚单位I基因的高变区V3-V4区域以及真核生物的18S核糖体RNA基因的高变区V3区域。扩增产物在Illumina® MiSeq测序仪上进行600个循环的配对端测序。本数据集包括解复用测序输出、具有分类注释的特征表以及样本元数据。