Metagenome for anaerobic digestion

PacBio long reads sequencing presents several potential advantages for DNA assembly and providing more complete gene profiling of metagenomic samples, however, lower single-pass accuracy can make gene discovery and assembly for low-abundance organisms difficult. To evaluate the application and performance of PacBio long reads and Illumina HiSeq short reads for metagenomics, we directly compared various assemblies with PacBio and Illumina sequencing reads on several anaerobic digestion microbiome samples.