Targeting S6K1 and S6K2 by siRNA and sgRNA in LN229 GBM cells

To systematically identify the function of S6K1 and S6K2 in glioblastoma cells, LN229 cells were stably transduced with Cas9, control non-targeting sgRNA (sgNT), or sgRNAs targeting S6K1 or S6K2. The cells were then transiently transfected with non-targeting siRNAs (siNT), or siRNA pools targeting S6K1 or S6K2. RNA sequencing was performed to compare cellular responses to inactivation of single or combination S6 kinases. Overall design: RNA-seq of LN229 GBM cells treated with either siS6K1, siS6K2, sgS6K1, sgS6K2, siS6K1 + sgS6K2, siS6K2 + sgS6K1