Arginase-driven eNOS uncoupling as the vascular lesion of chronic heat exposure in rats: dataset, statistical output and analysis code

Complete dataset, statistical output, analysis code and figures for the manuscript "Arginase-driven eNOS uncoupling as the vascular lesion of chronic heat exposure in rats" by Mohamed E. Elbeeh (Department of Biology, Jamoum University College, Umm Al-Qura University, 21955 Makkah, Saudi Arabia). STUDY. Male outbred Wistar rats (strain WI, RRID:RGD_13508588) were exposed to 38 +/- 1 C for 4 h/day, 5 days/week for 4 weeks, against thermoneutral controls at 24 +/- 1 C. Six four-week groups of n = 9 (thermoneutral vehicle, heat vehicle, heat + nor-NOHA, heat + L-citrulline, heat + vitamin C, thermoneutral + nor-NOHA) and two two-week interim vehicle subgroups of n = 6; 66 rats in total. The primary endpoint was acetylcholine-induced relaxation of mesenteric arterial rings in a wire myograph. Ethics approval HAPO-02-K-012-2026-01-2002, Biomedical Research Ethics Committee, Umm Al-Qura University, granted 1 January 2026. CONTENTS. The raw data workbook (18 sheets, 13,013 data rows), the main analysis script with its timestamped run log and complete statistical output as JSON, the companion analysis script with its output (log-scale remedy analysis, sensitivity analyses of the primary endpoint, cage-level food and water intake, two-week comparisons and derived indices), the nine populated result tables with ring-level myograph fits and animal-level tail-cuff session means, three supplemental tables with their legends, and the six figures at 300 dpi. Every number in the manuscript can be recomputed from these files; nothing in the analysis is hard-coded. A README file describes every sheet and column. NOTE ON VERSION 2 OF THE WORKBOOK. The primer sheet in the first version carried a placeholder amplification efficiency of 95 % for all thirteen genes, which contradicted the per-gene values measured from the dilution series and recorded in the qPCR sheet. Version 2 replaces those placeholders with the measured values (94.3 % for Nos2 to 99.5 % for Actb), adds melt-curve temperatures, and replaces Arabic annotations with English. No measurement value was changed. LIMITS OF THE DATASET. Each animal contributed one ring to each myograph protocol, so the ring value is the animal value and an animal-level random intercept is not identifiable. Several endpoints named in the analysis plan were never measured: the endothelium-denuded, indomethacin plus L-NAME, ex-vivo L-arginine and ODQ myograph protocols; mesenteric and hepatic arginase; plasma citrulline and symmetric dimethylarginine; tissue nitrite plus nitrate; aortic total antioxidant capacity; plasma and renal malondialdehyde; mesenteric gene expression; and urine osmolality, white-cell differential and plasma urea, creatinine, sodium and potassium. Aortic arginase activity and plasma HSP70 were assayed in the four-week cohort only. Nos2 was undetermined (Cq above 35) in all heat-exposed groups. These are absences in the dataset, not values withheld. Every biochemical, chromatographic, immunoassay and qPCR measurement was made in duplicate, and the animal value is the mean of the duplicates. Four four-week animals (one each in heat vehicle, heat + L-citrulline, heat + vitamin C and thermoneutral + nor-NOHA) were not included in the arginase, amino-acid/ADMA and immunoassay runs. VERSION 2 OF THE DEPOSIT. Adds the companion analysis script and its output, the supplemental material legends, Supplemental Table S3 with food and water intake analysed at cage level, and an updated Figure 1 giving the per-group n for the primary endpoint. No measurement value was changed. FUNDING. Umm Al-Qura University, Saudi Arabia, grant 26UQU4340520GSSR15.