Physiologic adaptation of Channichtydae during its development, focusing on expression and activity of the inducted form of nitric oxide syntase (NOS)

In English:

The campaign objective was to capture ice fishes
(Fam. Channichthyidae) larva to determine: - The different isoforms of
nitric oxide syntases, at proteines and RNAm, as molecular markers os
hipoxy.

- The larval development status by means of the age through the
otolites analysis and RNA/DNA rates.

- To determinate the oxygen comsumption under controlled
conditions.

A secondary objective was to study the ictioplankton
distribution regarding to the oceanographic parameters as well
as other biological aspects of it. Also the krill Euphasia superba
oxygen consumption was analysed at different development stages.

A network of oceanographic stations following a stratified
design was established, with 15 nautic miles of distance between
stations. In each station a vertical profile recording was done
the oceanographic parameters: salinity, conductivity,
temperature, chlorophyll, turbity, depth and oxygen, by means of
a CTD fitted to a Rosette with Niskin bottles. The bottles were
used to obtain samples of water at different depths. It was also
done fishing by Bioness multi-net whose allows us to obtain up
to 9 samples of plankton at controlled depths in real
time. Finally, the plankton sampling was completed with an IKMT
fishing. In some stations consecutive fishing were done to
obtain complementary material. In the locations where more ice
fishes were found the stations were repeated. In this way, in
Port Foster in Deception Island a total of 33 fishing were done
and the 14, 18, 22 and 25 stations were repeated. Finally a N-S
line with 11 stations separated 5 miles was completed with the
objective of study the plankton variations related to the water
masses existing in the Bransfield Strait.

En Espanol:

El objetivo de la campana era capturar larvas de peces de hielo
(Fam. Channichthyidae) para determinar:

- Las distintas isoformas de oxido nitrico
sintasas, al nivel de proteina y ARNm, como marcadores
moleculares de hiperoxia.

- El estado de desarrollo larvario mediante la asignacion de
la edad por medio del analisis de los otolitos y de las tasas de
ARN/ADN.

- Determinar el consumo de oxigeno en condiciones controladas

Como objetivo secundario se estudio la distribucion del
ictioplancton en relacion con los parametros
oceanograficos asi como otros aspectos biologicos del
mismo. Ademas, se analizo el consumo de oxigeno de las
distintas fases del desarrollo de krill Euphasia superba.

Se establecia una red de estaciones oceanograficas siguiendo un
diseno estratificado, con las estaciones separadas unas 15
millas nauticas. En cada estacion se realizo un perfil vertical
de los parametros oceanograficos: salinidad, conductividad,
temperatura, clorofila, turbidez, profundidad y oxigeno,
mediante una batisonda CTD acoplada a una Rossetta con botellas
Niskin. Las botellas se utilizaron para obtener muestras de agua
a profundidades determinadas. A continuacion se realizaron
pescas mediante una multired Bioness que permite obtener hasta 9
muestras de plancton a profundidades controladas a tiempo
real. Finalmente, se complemento el muestreo del plancton con
una pesca con IKMT. En algunas estaciones se realizaron varias
pescas consecutivas para obtener material
complementario. Ademas, en las localizaciones en que se
obtuvieron mas peces de hielo, se repitieron las
estaciones. Asi, en el Puerto Foster en la Isla Decepcion se
llevaron a cabo un total de 33 pescas y las estaciones 14, 18,
22 y 25 se repitieron. Finalmente, se realizo un transecto
norte-sur con 11 estaciones separadas 5 millas, con el objetivo
de estudiar la variacion del plancton en relacion con las masas
de agua que se encuentran en el Estrecho de Bransfield.