Effects of Conditional Egfr deletion on transcriptome of the mouse cerebral cortex

To investigate the role of Egfr in the cerebral cortex and hippocampal formation we conditionally deleted it using a floxed allele in combination with the Nestin-cre transgene. Cortices at E17.5 embryonic stage were harvested and split into rostral and caudal halves and RNA extracted and sequenced Comparison of RNA-seq data between rostral and caudal cortices and hippocampal formation of control brains (EgfrF/+ genotype) and brain-specific knock of Egfr (Nestin-EgfrF/F).为探究表皮生长因子受体（Egfr）在大脑皮层与海马结构中的作用，我们采用两侧带有loxP位点的等位基因（floxed allele）结合Nestin-cre转基因（Nestin-cre transgene）对其实施条件性敲除。收集胚胎发育第17.5天（E17.5）的大脑皮层样本，将其分为吻侧与尾侧两半，随后提取RNA并进行RNA测序（RNA-seq）。对基因型为EgfrF/+的对照组小鼠，以及大脑特异性敲除Egfr的Nestin-EgfrF/F小鼠的吻侧皮层、尾侧皮层及海马结构的RNA测序数据开展对比分析。