The lncRNA locus Handsdown regulates cardiac gene programs and is essential for early mouse development

WT and Handsdown mutant ESC were differentatied into the meosdermal cell lineage and harvested at desiganted timepoints für differential expression profiling (n=3). Hearts of E9.5 mouse embryos were harvested and profiled to generate a represantative picture of RNAs (n=1). 4C-seq analysis of mESCs, cardiomyocytes and whole hearts from E11.5 mouse embryos Overall design: Examination of 2 differnet chromatin states and RNA expression in 2 different Brachyury mutants embryos of in vitro differentiated mouse embryonic stem cells 4C-seq with the promoter region of Handsdown as the viewpoint