Three potential off-target sites for each gRNA were identified by CRISPOR (http://crispor.org) based on the protospacer adjacent motif (PAM) sequence in the hg38 reference genome and a high mitOfftarg
Vector capturing was used for experimental identification of potential off-target sites of 2 gRNA targeting alpha globin genes Overall design: K562 cells were transduced with an Integrase-defective le
induced pluripotent stem cells (iPSCs) underwent homology directed repair using the CRISPR/Cas9 genome editing system in the presence of different small molecules. Amplicon sequencing was then perform
