RNA-seq of endothelial cells from 28 hours post fertilization sf3b1 mutant and sibling zebrafish with Stat3 overexpression

The purpose of the experiment was to define the gene expression and splicing alterations occurring when wildtype or consitutively active Stat3 are expressed in embryonic zebrafish endothelial cells with low levels of splicing factor 3b subunit 1 (Sf3b1). Results provide insight the interplay of Sf3b1 and Stat3 in hematopoietic development. Overall design: mRNA profiling of kdrl-positive endothelial cells isolated from 28 hours post fertilization wildtype, sf3b1 mutant and sibling zebrafish with wildtype or constitutively active Stat3 overexpression using the Novogene 6000 platform. A total of 15 samples were sequenced.