Infiltration of GFP+ BM-derived cells into the spinal cord in WT/TRPM2-KO BM chimeric mice.

(A, B) GFP+ cells and Iba1+ cells were visualized by GFP fluorescence (green) and immunostaining with Iba1 antibody (red), respectively, in the spinal cord sections 14 days after pSNL surgery. Iba1+/GFP+ cells were visualized as a yellow signal in merged images. (A) Representative microphotographs in WT-BM and WT chimeric mice are shown (scale bars = 200 µm). (B) Representative microphotographs in selected regions of contralateral and ipsilateral spinal dorsal horn (defined by the rectangular area in A) are shown (scale bars = 100 µm). (C) The numbers of Iba1-/GFP+ cells, Iba1+/GFP-cells, and Iba1+/GFP+ cells within the selected regions were counted in the contralateral (left panel) and ipsilateral (right panel) spinal dorsal horn. n = 3–6. Data are expressed as the mean ± SEM.