Methyl-SNP-seq reveals dual readouts of methylome and variome at molecule resolution

We developed Methyl-SNP-seq, a technology that takes advantage of the redundancy of the double helix, to extract the methylation and sequence information from a single original DNA molecule We demonstrate the usefulness of this technology on a broad spectrum of applications ranging from allele specific methylation analysis in humans to methylation identification in complex bacterial communities. Overall design: Methyl-SNP-seq uses bisulfite conversion of one of the double strands to identify methylation while the other strand is left intact for sequencing. Both strands are locked together to link the dual readout on a single paired-end read.