Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis (CBX148). unidentified

The analysis was performed with the Agilent Arabidopsis 3 Oligo Microarray for 44K Microarray analysis (Agilent Technologies). Samples of MM2d cells were biologically duplicated, and total RNA was extracted using the NucleoSpin RNA Plant (MACHEREY-NAGEL) as described in the manufacturer?fs instructions. Each RNA sample was labeled for Cy3-cRNA probes, according to the instructions for one color experiment. The hybridized and washed material on each glass slide was scanned by an Agilent DNA microarray scanner (model G2505B; Agilent Technologies). The location and delineation of every spot in the array were established by Feature Extraction Software version 9.5.3.1 (Agilent Technologies). Fluorescence intensity was integrated through filtration and normalization, and the p-value of each spot was calculated using default parameters. Two biological replicates were analyzed.