Single cell RNA-seq for human and bovine PBMCs stimulated with M. tuberculosis. Single cell RNA-seq for human and bovine PBMCs stimulated with M. tuberculosis

We propose a custom pipeline for combining data from experiments on multispecies studies tested on data from human and bovine peripheral blood mononuclear cellssamples, stimulated with Mycobacterium tuberculosis. Overall design: PBMC samples from cattle and human were isolated using pancoll (Pan-Biotech) gradient density centrifugation following the manufacturer’s protocol and analysed using scRNAseq.