Changes in gut commensal composition with age in early injured and untreated groups of flies under co-culture conditions

Female flies(<i>esg-Gal4&gt;GFP</i>) from untreated (UT) and early injury (EI) groups were mixed in the same cage. At 16 and 24 days of age, 20 midguts from untreated (UT) and early injured (EI) flies were dissected in 1x sterile PBS solution and commensal genomic DNA was extracted using the TIANamp Genomic DNA Kit (TIANGEN Biotech, Cat# DP304-02). The 16s rDNA sequencing library construction and sequencing process were performed by Majorbio Bio-Pharm Technology Co. Ltd (Shanghai, China) (https://www.majorbio.com/).Three sample: UT 16d (1,2,3), EI 16d (1,2,3) of each group were tested independentlyS16 represents the gut microbiota composition of the UT group (with 5% sucrose) on day 16.B16 represents the gut microbiota composition of the EI group (with Bleomycin) on day 16.Four sample: UT 24d (1,2,3,4), EI 24d (1,2,3,4) of each group were tested independentlyS24 represents the gut microbiota composition of the UT group (with 5% sucrose) on day 24.B24 represents the gut microbiota composition of the EI group (with Bleomycin) on day 24.<br>