miRNA sequence analysis of primary lesions, brain metastases, and normal lung tissues in lung adenocarcinoma

We obtained primary lesions, brain metastases, and normal lung tissues from lung adenocarcinoma patients. Next generation sequencing was performed to assess the expressions of miRNA in these specimens. Overall design: A total of 15 samples (5 normal lung tissues, 5 primary tumors, and 5 brain metastases) were analyzed using small RNA sequencing technology to generate a miRNA expression signature.