Mapping translocation breakpoint using Solexa sequencing

We have performed shotgun sequencing of flow-sorted derivative chromosomes using next generation (Solexa/Illumina) multiplex sequencing-by-synthesis technology. As shown here for three different disease-associated BCRs, the coverage attained by this platform is sufficient to bridge the breakpoints by PCR amplification, and this procedure allows to determine their exact nucleotide positions within few weeks